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KMID : 0613820040140040608
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Journal of Life Science
2004 Volume.14 No. 4 p.608 ~ p.613
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Optimization of Culture Conditions for the Production of Pyrimidine Nucleotide N-Ribosidase from Pseudomonas oleovorans
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Yoo Dae-Sik
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Abstract
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Pyrimidine nucleotide N-ribosidase (pyrimidine 5’-nucleotide phosphoribo (deoxyribo)hydrolase/pyrimidine 5’-nucleotide nucleosidase, EC 3.2.2.10) directly catalyzes pyrimidine 5’-nucleotide to pyrimidine base and ribose (deoxyribo) 5-phosphate. In order to clarify the best nutritional conditions for the growth and the pyrimidine nucleotide N-ribosidase production of Pseudomonas oleovorans ATCC 8062 the effects of various nutrients such as different carbon and nitrogen sources were studied. For the both the growth and the enzyme production, 2% fumarate, 1.5% peptone, 5% corn steep liquor (CSL) and 1% ammonium chloride were excellent carbon and nitrogen sources, respectively. Optimum pH, temperature, and cultivation time for the enzyme production were 7.0, 28℃, and 48 h, respectively. The pyrimidine nucleotide N-ribosidase of P. oleovorans ATCC 8062 was not induced by UMP and its derivatives, and was constitutive enzyme.
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KEYWORD
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Pyrimidine nucleotide N-ribosidase, Pseudomonas oleovanans, pyrimidine base
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